Rapid panoramic mapping in NanoSP

The NanoSP software system is intended for acquisition and processing of spectral data when working with confocal scanning microscopes manufactured by SOL instruments.

NanoSP provides: complete control of all automated units and modules of the confocal microscope; performing spectral measurements at a point on the sample; automated spectral calibration with a built-in light source; background subtraction; acquiring one, two and three-dimensional maps of the sample using the reflected laser light and Raman scattering signal; as well as further data processing and analysis. We present NanoSP as a demonstration of our capabilities and experience, if you are interested in development of a similar system for your equipment and devices, please contact us!

Functionality of NanoSP

  • Control of all automated units and modules of the microscope: starting the device, checking its status and adjusting the parameters; setting the optimal parameters for recording spectral information; an additional level of parameters customization of the scanning microscopes-spectrometers for advanced users.
  • Performing spectral measurements at a point on the sample: spectra detection in various acquisition modes; acquiring panoramic spectra; displaying multiple spectra on the same graph; peak tracer; converting spectra into different formats.
  • Automated spectral calibration with a built-in light source: built-in compact calibration light source with known reference lines allows for the best accuracy in determining the positions of spectral peaks.
  • Background subtraction: background subtraction for several spectra simultaneously, baseline correction, spectrum normalization, and spectrum smoothing.
  • Spectral edge analysis: the ability to divide Raman bands into different spectral components (to perform fitting).
  • Acquiring spectral images of objects: fast scanning; full Raman spectra saving in every point; creating the color-coded maps which show molecular species distribution.
  • Rapid panoramic mapping of a large sample area using a galvanic mirror scanner and an automated stage.
  • Metrical and statistical image analysis: measuring distances and areas; finding the minimum, maximum, standard deviation, mass center, and other image statistics.
  • Image processing: variety of functions for mathematical processing; convenient presentation of the results with coloring; digital filtering; working with histograms.
  • Axonometric representation of images: representation of the image in the form of a three-dimensional surface.
  • Section view: the ability to easily obtain any section and present the results in a convenient form; cross-section analysis.
  • Program interface: separate pages for working with spectra and images; separate panels for working with built-in functions; easy access to frequently used parameters; pop-up hints; detailed reference system for user convenience.
  • Automated focusing on a sample surface with reflected unit: when switching between objectives, NanoSP ensures that you are focused; automated focusing on the sample surface is performed using the reflected laser light.
  • Mathematical operations with spectra and images: addition, subtraction; correction for cosmic ray emissions; smoothing, normalization; and similar operations.
  • Advanced capabilities for working with spectra: the program allows to display spectra in a convenient way, customize the style and color; the spectra buffer allows to compare spectra obtained at different times, or from different parts of the detector matrix.
  • Saving experiment configurations and program settings.
  • Linking to the Raman spectral databases: working with the spectral database (spectrum identification is possible using advanced capabilities of the LabCognition software package); ability to create your own spectral libraries; spectrum interpretation; functional groups search using Raman bands.
  • Optional chemometric software package.
  • 3D data visualization and analysis: three-dimensional representation; all kinds of sections; resizing and rotating images; digital filtering; presentation in various colors; three-dimensional view with varying degrees of transparency.

Functional capabilities of NanoSP

  • Control of all automated units and modules of the microscope: starting the device, checking its status and adjusting the parameters; setting the optimal parameters for recording spectral information; an additional level of parameters customization of the scanning microscopes-spectrometers for advanced users.
  • Performing spectral measurements at a point on the sample: spectra detection in various acquisition modes; acquiring panoramic spectra; displaying multiple spectra on the same graph; peak tracer; converting spectra into different formats.
  • Automated spectral calibration with a built-in light source: built-in compact calibration light source with known reference lines allows for the best accuracy in determining the positions of spectral peaks.
  • Background subtraction: background subtraction for several spectra simultaneously, baseline correction, spectrum normalization, and spectrum smoothing.
  • Spectral edge analysis: the ability to divide Raman bands into different spectral components (to perform fitting).
  • Acquiring spectral images of objects: fast scanning; full Raman spectra saving in every point; creating color-coded maps which show molecular species distribution.
  • Rapid panoramic mapping of a large sample area using a galvanic mirror scanner and an automated stage.
  • Metrical and statistical image analysis: measuring distances and areas; finding the minimum, maximum, standard deviation, mass center, and other image statistics.
  • Image processing: variety of functions for mathematical processing; convenient presentation of the results with coloring; digital filtering; working with histograms.
  • Axonometric representation of images: representation of the image in the form of a three-dimensional surface.
  • Section view: the ability to easily obtain any section and present the results in a convenient form; cross-section analysis.
  • Program interface: separate pages for working with spectra and images; separate panels for working with built-in functions; easy access to frequently used parameters; pop-up hints; detailed reference system for user convenience.
  • Automated focusing on a sample surface with reflected unit: when switching between objectives, NanoSP ensures that you are focused; automated focusing on the sample surface is performed using the reflected laser light.
  • Mathematical operations with spectra and images: addition, subtraction; correction for cosmic ray emissions; smoothing, normalization; and similar operations.
  • Advanced capabilities for working with spectra: the program allows to display spectra in a convenient way, customize the style and color; the spectra buffer allows to compare spectra obtained at different times, or from different parts of the detector matrix.
  • Saving experiment configurations and program settings.
  • Linking to the Raman spectral databases: working with the spectral database (spectrum identification is possible using advanced capabilities of the LabCognition software package); ability to create your own spectral libraries; spectrum interpretation; functional groups search using Raman bands.
  • Optional chemometric software package.
  • 3D data visualization and analysis: three-dimensional representation; all kinds of sections; resizing and rotating images; digital filtering; presentation in various colors; three-dimensional view with varying degrees of transparency.