NanoSP – software for confocal microscopy
NanoSP – software for confocal microscopy
Software for Confotec® microscopes

The NanoSP software system is designed for acquisition and processing of spectral data when working with confocal scanning microscopes of the Confotec® series manufactured by SOL instruments. NanoSP provides: complete control of all automated units and modules of the microscope, performing spectral measurements at a point on the sample, automated spectral calibration with a built-in light source, background subtraction, acquisition of one/two/three-dimensional maps of the sample, as well as further data processing and analysis. Below we present the NanoSP features as a demonstration of our skills and experience. If you are interested in development of a similar software for your equipment, contact us!

Software for Confotec® microscopes

The NanoSP software system is designed for acquisition and processing of spectral data when working with confocal scanning microscopes of the Confotec® series manufactured by SOL instruments. NanoSP provides: complete control of all automated units and modules of the microscope, performing spectral measurements at a point on the sample, automated spectral calibration with a built-in light source, background subtraction, acquisition of one/two/three-dimensional maps of the sample, as well as further data processing and analysis. Below we present the NanoSP features as a demonstration of our skills and experience. If you are interested in development of a similar software for your equipment, contact us!

Control of all automated units and modules of the confocal microscope: starting the device, checking its status and adjusting the parameters, setting the optimal parameters for recording spectral information, and additional customization of parameters for advanced users.

Performing spectral measurements at a point on the sample: spectra detection in various acquisition modes, acquisition of panoramic spectra, displaying multiple spectra on the same graph, peak tracer, converting spectra into many different formats.

Automated spectral calibration with a built-in light source: when using a built-in compact calibration light source with known reference lines, NanoSP allows you to achieve maximum accuracy in determining the positions of spectral peaks.

Background subtraction and spectral edge analysis: background subtraction for several spectra simultaneously, baseline correction, spectrum normalization, spectrum smoothing, the ability to divide Raman bands into different spectral components (to perform fitting).

Acquiring spectral images of objects: fast mapping; saving Raman spectra at each image point; generation of distribution maps of molecular compounds; overlay of color-coded Raman images showing the distribution of individual molecular complexes.

Connection with Raman spectral databases: working with spectral databases, identification of chemical compounds using the LabCognition software, creation of custom spectral libraries, interpretation of spectra, search for functional groups based on Raman peaks.

3D data visualization and axonometry: 3D representation, all kinds of sections, resizing and rotating images, digital filtering, coloring, 3D view with different degrees of transparency, and axonometric representation (image as a three-dimensional surface).

Анализ спектральных контуров
Spectral edge analysis
Спектральные измерения в отдельной точке
Spectral measurements at a point
Аксонометрическое представление изображений
Axonometric representation
Сечения трехмерной модели
Sections of a 3D model
Spectral edge analysis
Spectral edge analysis
Sections of a 3D model
Sections of a 3D model
When switching between objectives, NanoSP performs automated focusing on the sample surface using reflected laser light.
Rapid panoramic mapping of a large sample area using a galvanic mirror scanner and a motorized stage.
Many functions for mathematical image processing, convenient presentation of the results with coloring, digital filtering, working with histograms, measuring distances and areas, and finding various image statistics.
Program interface: separate pages for working with spectra and images, separate panels for working with built-in functions, easy access to frequently used parameters, pop-up hints, detailed reference system for user convenience, and saving experiment configurations and program settings.
Addition, subtraction, correction for cosmic ray emissions, smoothing, normalization, and similar mathematical operations with spectra.
NanoSP allows to display spectra in a convenient way, customize the style and color; the spectra buffer allows to compare spectra obtained at different times or from different parts of the detector matrix.
Optional chemometric software package.
Advanced setup of the spectral device and detector parameters: all registration parameters are configured on a separate page, averaging and summation are implemented, as well as external and internal synchronization.